2016 Fiscal Year Final Research Report
Ecological function of wood insect associated microbes
| Project/Area Number |
15K18812
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental agriculture(including landscape science)
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 木質成分分解性微生物 / プロテオミクス |
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| Outline of Final Research Achievements |
In this study, we attempted to identify the woodwasp symbiont microbes from Hokkaido forest. We captured two wood wasp species, Sirex nitobei and Xiphydria cannelus. One bacteria and one fungus were isolated from a mygcangia of X. canelus, and were identified as Rahnella spp., and Daldinia decipiens species by 16s rRNA and ITS sequencing, respectively. Furthermore, we determined the hemicellulose-degrading activity in the supernatant when D. decipiens was cultured in the liquid medium containing polysaccharides such as cellulose, hemicellulose, wood biomass as sole carbon source, and glucose was used as a control culture. By utilizing proteomics, we were able to determined multiple glycoside hydrolase family enzymes, which are predicted to break down various polysaccharides present in woody biomass.
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| Free Research Field |
生化学・分生生物学
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