Why is the spindle assembly checkpoint weak in oocytes?

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K18823
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Hirohisa Kyogoku 国立研究開発法人理化学研究所, 多細胞システム形成研究センター, 訪問研究員 (20726038)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 卵母細胞 / 紡錘体形成チェックポイント / ライブイメージング / マウス / 減数分裂 / 顕微操作

Outline of Final Research Achievements

It is known that the spindle assembly checkpoint is less stringent in oocytes than in somatic cells. In this study, using live imaging and micromanipulation, we show that cytoplasmic size affects the efficiency of spindle assembly checkpoint. Moreover, we found that the cytoplasmic size-dependent dilution of nuclear factors, limits spindle assembly checkpoint efficiency. These data suggested that the stringency of the spindle assembly checkpoint is limited by the cytoplasmic/nucleus ratio, which explain why the spindle assembly checkpoint is weak in the large volume of oocytes.

Free Research Field

繁殖生物学