Elucidation of the molecular mechanism of heavy metal tolerance and accumulation in copper moss by transcriptome analysis and genome editing technology

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K18824
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Nomura Toshihisa 国立研究開発法人理化学研究所, 環境資源科学研究センター, 特別研究員 (20722771)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ホンモンジゴケ / 重金属耐性 / コケ植物 / ゲノム編集 / CRISPR/Cas9システム

Outline of Final Research Achievements

In order to develop technologies for heavy metal phytoremediation, it is important to elucidate the heavy metal tolerance and accumulation mechanism of the hyperaccumulator plants. In this study, we focused on copper mosses Scopelophila cataractae which has copper tolerance and accumulation ability. By using genomic editing technology and transcriptome analysis, we aimed to elucidate the molecular mechanisms underlying copper tolerance and accumulation in S. cataractae. As a result, we found that the mechanism of copper export from intracellular to extracellular via copper transporter ScHMA5 is necessary for copper tolerance ability in S. cataractae.

Free Research Field

植物細胞分子生物学