2016 Fiscal Year Final Research Report
In situ photopolymerization of polyacryrlamide gels for specific entrapment and analysis of carbohydrates using microchip electrophoresis
| Project/Area Number |
15K18852
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | Kindai University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | マイクロチップ電気泳動 / 糖鎖 / オンライン濃縮 / 酵素消化 |
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| Outline of Final Research Achievements |
For the fast releasing of N-linked glycans from glycoproteins, a simple and efficient method has been developed by fabricating trypsin and peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase (PNGase F)-impregnated polyacrylamide gel onto a commercial pipette tip, respectively. The fixed enzymes maintained their activities on the polyacrylamide gel and the reaction was completed by a few times pipetting operation.Also, a simple and efficient method has been developed to fabricate sample concentrate and derivative on a channel of a commercial polymethylmethacrylate-made microchip The availability of ionic acrylamide gel was demonstrated for the sensitive analysis of some FITC-amino acids under various buffer systems at several different pH. Then, the multi channel microchip was developed for combination of various functions of acrylamide gel, and achieves high-throughput glycans analysis.
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| Free Research Field |
分析化学
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