Transcriptional regulatory mechanism of the RNA polymerase II initiation complex by the chromatin remodeling complex

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K18861
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: University of Toyama
• Principal Investigator: Tanaka Aki 富山大学, 大学院医学薬学研究部(薬学), 助教 (50432109)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 遺伝子発現 / 転写 / 基本転写因子

Outline of Final Research Achievements

We performed Mass spectrometry to identify TFIIEα binding proteins. It was indicated that TFIIEα interacted with the SWI/SNF complex which is one of the chromatin remodeling complexes. The SWI/SNF complex can be divided into BAF and PBAF. Our immunoprecipitation study demonstrated that TFIIE interacted with the PBAF mainly and TFIIH. TFIIE recruits TFIIH to complete the PIC formation and regulates enzymatic activities of TFIIH. There is an interesting possibility that TFIIE-TFIIH act on the promoter coordinately with the PBAF complex and regulates transcription and the chromatin structure together.

Free Research Field

医歯薬学