2016 Fiscal Year Final Research Report
Cloning, expression, purification, and characterization of enzymes that cleave puerarin C-glucosidic bond.
| Project/Area Number |
15K18891
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Natural medicines
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| Research Institution | Suzuka University of Medical Science |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | C-配糖体 / 腸内細菌 / プエラリン / 代謝酵素 |
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| Outline of Final Research Achievements |
Puerarin is an isoflavone C-glucoside contained in the roots of Pueraria lobata Ohwi. We previously isolated a human intestinal bacterium, called strain PUE, which can metabolize puerarin to daidzein. In this study, cloning, expression, purification, and characterization of enzymes that cleave puerarin C-glucosidic bond from strain PUE were accomplished.
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| Free Research Field |
天然物化学
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