2016 Fiscal Year Final Research Report
Development of SIRT6 activators targeting for Warburg effect
| Project/Area Number |
15K18899
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Drug development chemistry
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| Research Institution | Nagoya City University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | Sirtuin / 蛍光プローブ |
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| Outline of Final Research Achievements |
In general, sirtuin activity is detectable by a protease (eg. trypsin) coupled two-steps method. However, such method has several problems, cumbersome procedure, false-positive compounds on chemical screening.
In this study, focusing on interesting reports showing that sirtuin recognize and hydrolize long-chain fatty acyl groups, we hypothesized that sirtuin can hydrolize acyl groups of large quencher dyes. We exhibited that sirtuin activity is detectable by a FRET based novel fluorescence probe, SFP3, and applied this methodology for chemical screening. We identified novel scaffolds of SIRT6 inhibitor and activiator.
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| Free Research Field |
ケミカルバイオロジー
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