Isolation of human iPS-cell derived intestinal stem cells and constitution of pharmacokinetic evaluation system

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K18929
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical pharmacy
• Research Institution: Nagoya City University
• Principal Investigator: Iwao Takahiro 名古屋市立大学, 大学院薬学研究科, 准教授 (50581740)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: iPS細胞 / 小腸 / 幹細胞 / 薬物動態

Outline of Final Research Achievements

This study indicated to be possible to isolate and maintain human iPS cell-derived intestinal cells. The isolated cells were intestinal stem cell-like cells and had a capacity of differentiation to the enterocytes. The maintained cells remained having a stemness after a certain period of a maintenance culture. When the maintained cells were differentiated to the enterocytes, the cells had pharmocokinetic functions such as CYP3A4 activity, CYP3A4 inducibility, and transporter activities of efflux transporters. It was thought that these results could be useful technical information to use of human iPS cell-derived enterocytes in drug development studies.

Free Research Field

薬物動態学