2016 Fiscal Year Final Research Report
Developmental and pathologic alteration of podocyte 3D structure
Project/Area Number |
15K18960
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
General anatomy (including histology/embryology)
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Research Institution | Juntendo University |
Principal Investigator |
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Keywords | 足細胞 / ポドサイト / スリット膜 / 細胞間結合装置 / 三次元超微形態解析 / FIB/SEM / 糸球体発生 / ネフローゼ症候群 |
Outline of Final Research Achievements |
Podocytes are well known to have a unique 3D architecture specialized for glomerular filtration. However, several 3D morphological aspects on podocyte planarization and degeneration remain fragmentary because they are difficult to reveal by conventional scanning electron microscopy. Here, we adopted FIB/SEM tomography, a powerful tool for analyzing the three-dimensional cellular ultrastructure, to precisely reveal the morphological process of podocyte development and the morphological process of podocyte planarization in puromycin aminonucleotide (PAN) nephrosis rats, an animal model for human minimal change nephrotic syndrome.
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Free Research Field |
解剖学、三次元超微形態解析
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