2017 Fiscal Year Final Research Report
Investigating the role of a conventional marker protein in ORNs
| Project/Area Number |
15K18967
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | Kurume University (2016-2017)
Kyoto University (2015) |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | ヌクレオチド / イオンチャネル / 神経細胞 / 自発発火 / 行動生理学 |
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| Outline of Final Research Achievements |
Neurons and cells often express various Gαs-protein-coupled receptors (GPCRs), many of which show ligand-independent basal activity. HCN2 channels opens in response to the basal cAMP pool size produced by basally active GPCRs. Here, we utilized an exogenous HEK293 expression system and voltage-clamp patch-clamp recordings to investigate basal HCN2 channel activity in the presence of two GPCRs with diverse basal activities. We used the β2-adrenoceptor (β2AR) together with odorant receptors (ORs), as both GPCR families are known to show strong basal activity. We found that β2AR alone strongly enhanced the activity of HCN2 channels, and co-expression of ORs further diversified the HCN2 channel activity, which was abolished by an adenylate cyclase inhibitor.
Olfactory marker protein shows the capability to modulate the basal nucleotide pool. We demonstrated the actions by employing the knock-out mice (under review for publication).
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| Free Research Field |
神経生理学
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