Glucose Fluctuations aggravate diabetic cardiomyopathy

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K18972
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: Oita University
• Principal Investigator: Saito Shotaro 大分大学, 医学部, 病院特任助教 (60631380)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 糖尿病 / 血糖変動 / 酸化ストレス / 虚血再灌流障害

Outline of Final Research Achievements

Male rats were randomly assigned to either a control, DM, and DM with GF group. DM was induced by an injection of streptozotocin, and glucose fluctuation was induced by starvation and insulin injection. One sequential program comprised 2 hypoglycemic episodes during 4 days. The isolated hearts were subjected to 20-min ischemia/30-min reperfusion. The infarct size was larger in hearts with GF than those with sustained hyperglycemia. Activities of catalase and superoxide dismutase were decreased, and expressions of NADPH oxidase and thioredoxin-interacting protein were upregulated by GF accompanied by an increase of reactive oxygen species (ROS). Swollen mitochondria with destroyed cristae were observed in diabetic hearts; they were further devastated by GF. Microarray analysis revealed that the expressions of miRNA-200c and miRNA-141 were abundant in those hearts with GF.

Free Research Field

循環器内科