2016 Fiscal Year Final Research Report
Role of SIRT1 in mitophagy in the skeletal muscle
| Project/Area Number |
15K18992
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General pharmacology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | マイトファジー / SIRT1 / オートファジー |
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| Outline of Final Research Achievements |
We found skeletal muscle dysfunction and impaired activation autophagy in skeletal muscle-specific SIRT1 knockout mice. These suggest that SIRT1 in skeletal muscle cells plays an important role in maintaining skeletal muscle function via autophagy. In myoblasts, resveratrol (RSV), a SIRT1 activator, promoted mitophagy. Mitochondrial reactive oxygen species (ROS) levels induced by antimycin A were significantly reduced by RSV. This effect of RSV was blocked by knockdown of PINK-1 which triggers mitophagy, suggesting that SIRT1 activation decrease mitochondrial ROS levels via mitophagy. We demonstrate using in vivo and in vitro models the relationship between SIRT1 and autophagy/mitophagy and its significance in skeletal muscle protection.
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| Free Research Field |
医歯薬学
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