2016 Fiscal Year Final Research Report
Elucidating the mechanisms underlying linear ubiquitin chains-mediated cell death protection
| Project/Area Number |
15K19007
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
General medical chemistry
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Keywords | 細胞死 / LUBAC / 直鎖状ポリユビキチン鎖 |
|---|
| Outline of Final Research Achievements |
LUBAC is involved in TNF-alpha mediated NF-kappaB activation via generating linear polyubiquitin chains. We discovered that LUBAC is also involved in the protection of TNF-alpha-mediated cell death in NF-kappaB independent manner. To elucidate the mechanisms, I prepared LUBAC knockout cells and reconstituted the several LUBAC mutants into cells to analyze whose domains are involved in cell death protection. Finally, we found a domain of LUBAC which participates in cell death regulation and explored proteins which bind to newly discovered domain by Mass spectrometry.
|
| Free Research Field |
分子生物学
|
