Global analysis of the molecular basis of epigenetic reprogramming in primordial germ cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K19008
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Kyoto University
• Principal Investigator: NAKAKI Fumio 京都大学, 医学研究科, 助教 (60737120)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 始原生殖細胞 / 胚性幹細胞（ES細胞） / 転写因子 / ChIP-seq / 発生・再生

Outline of Final Research Achievements

Primordial germ cells, the origin of germline cells, undergo epigenetic reprogramming in which their epigenetic modifications dramatically change just after their specification in mice. To elucidate the molecular basis of it, the roles of three transcription factors, BLIMP1, PRDM14, and TFPA2C were analyzed on a genome-wide scale. By utilizing an in vitro culture system that is functionally validated, ChIP-seq (chromatin immunoprecipitation followed by high-throughput DNA sequencing) and transcriptome analyses were conducted. These analyses suggested that both of BLIMP1 and PRDM14 played a pivotal role in the activation of germline program, and TFAP2C contributed to activation of transcription.

Free Research Field

分子生物学