Tyrosine phosphorylation of a viral protein in the hepatitis C virus life cycle

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K19021
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathological medical chemistry
• Research Institution: University of Fukui
• Principal Investigator: Yamauchi Shota 福井大学, 学術研究院医学系部門, 特命助教 (00728941)
• Research Collaborator: SADA Kiyonao 福井大学, 学術研究院医学系部門, 教授 (10273765) ; CHIHARA Kazuyasu 福井大学, 学術研究院医学系部門, 准教授 (00314948) ; TAKEUCHI Kenji 福井大学, 学術研究院医学系部門, 助教 (40236419) ; HOTTA Hak 神戸大学, 大学院医学研究科, 教授 (40116249)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: C型肝炎ウイルス / インターフェロン

Outline of Final Research Achievements

More than 1.5 million people are infected with hepatitis C virus (HCV) in Japan. Following entry into the host hepatocyte, the RNA genome of HCV is translated into viral proteins and replicated by the viral proeins. Viral RNA and proteins are assembled into virus particles, which are released from the host hepatocyte through the secretory pathway and infect other hepatocytes. In this study, we found that tyrosine phosphorylation of the HCV NS5A protein by c-Abl promotes virus particle assembly. We also reveal the difference between the signaling mechanisms of the antiviral cytokines inteferon (IFN)-alpha and IFN-lambda.

Free Research Field

生化学　ウイルス学