Spatial proximity of PATRRs and their translocation frequencies

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K19042
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Human genetics
• Research Institution: Fujita Health University
• Principal Investigator: KATO TAKEMA 藤田保健衛生大学, 総合医科学研究所, 助教 (20387690)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: PATRR / 染色体転座

Outline of Final Research Achievements

To clarify the mechanism of the PATRR-mediated translocation, I attempted to establish comprehensive PATRR-mediated translocations detection system using normal male sperm DNA as a template. Genome walking methods and hybridization capture for PATRR sequence enrichment system were used to identify unknown PATRRs and PATRR-mediated translocations. However these methods are limited to minimize nonspecific sequences. Therefore, I could not established the high sensitivity detection system of unknown PATRRs and translocations using these methods. Comprehensive PATRR and PATRR-mediated translocation detection system remains a future subject. I also attempted to establish single cell comprehensive chromosome analysis system using whole genome amplification and low coverage whole genome sequence by next generation sequencing. This system enable to analyze chromosome copy number alterations of individual germ cells. This result might lead to a chromosome copay number analysis in the future.

Free Research Field

細胞遺伝学