2016 Fiscal Year Final Research Report
Detection of SYT-SSX fusion gene product in tissue section.
Project/Area Number |
15K19051
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Human pathology
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Research Institution | Gifu University |
Principal Investigator |
Saigo Chiemi 岐阜大学, 大学院医学系研究科, 助教 (10547681)
|
Project Period (FY) |
2015-04-01 – 2017-03-31
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Keywords | 滑膜肉腫 / 特異抗体 |
Outline of Final Research Achievements |
Synovial sarcoma is an aggressive sarcoma with specific reciprocal chromosomal translocation of SS18 (also known as SYT) and SSX genes. In the present study, we aimed to detect the SS18-SSX fusion gene product in routinely processed pathological synovial sarcoma tissue section. Monoclonal antibodies to amino acids peptide, which covered the fusion region of SS18-SSX, were newly established and subsequently characterized by Enzyme-Linked ImmunoSorbent Assay, Western-immunoblotting, and Immunohistochemical staining. We generated over thirty antibodies, which immunostained SS18-SSX gene product in routinely processed formalin-fixed and paraffin-embedded tissue section; however, most antibodies exhibited the cross reactivity with SS18 and/or SSX proteins as well as SS18-SSX chimera protein. By contrast, a monoclonal antibody designated AS1SS, preferably reacted with boundary region of SS18-SSX chimera protein.
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Free Research Field |
人体病理学
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