2016 Fiscal Year Final Research Report
Detection of specific antibody-producing cells with the enzyme-labeled antigen method in paraffin sections
| Project/Area Number |
15K19063
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Fujita Health University |
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Principal Investigator |
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| Research Collaborator |
TSUTSUMI Yutaka
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| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Keywords | 酵素抗原法 / 抗体 / 抗原 / 病変局所 / 形質細胞 / パラフィン切片 |
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| Outline of Final Research Achievements |
The purpose of the present study was the application of the enzyme-labeled antigen method in formalin-fixed paraffin-embedded (FFPE) tissue sections. This purpose has not been achieved during this period. However, we elucidated factors inactivating antibodies during paraffin sectioning for the enzyme-labeled antigen method. Fixation methods maintaining antibody activities in paraffin sections were found. We demonstrated properties of paraffin wax used for embedding tissues involve antibody activities in tissue sections. These findings would help us to realize the application of the enzyme-labeled antigen method in FFPE sections.
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| Free Research Field |
人体病理学
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