2016 Fiscal Year Final Research Report
Analysis of release of mosquito-borne flaviviral particles
| Project/Area Number |
15K19069
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Experimental pathology
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | フラビウイルス / メンブレントラフィック / リサイクリングエンドソーム / 人獣共通感染症 |
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| Outline of Final Research Achievements |
We performed siRNA-based screening for silencing 18 Rab genes that are related to the vesicle transport from the ER to the plasma membrane. The siRNA screen revealed that KD of Rab8b significantly decreased VLP release efficiency compared with control siRNA-transfected cells. RNAi analysis revealed that Rab8b plays a role in WNV particle release. The amount of WNV particles in the supernatant of Rab8b-deficient cells was significantly decreased compared with that of wild-type cells. We also demonstrated that WNV particles accumulated in the recycling endosomes in WNV-infected cells. In summary, these results suggest that Rab8b is involved in trafficking of WNV particles from recycling endosomes to the plasma membrane.
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| Free Research Field |
基礎医学
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