Development of mutator Plasmodium falciparum and its application for mutants generation.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K19089
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Parasitology (including sanitary zoology)
• Research Institution: Tokyo Women's Medical University
• Principal Investigator: Honma Hajime 東京女子医科大学, 医学部, 助教 (10617468)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: マラリア / Plasmodium falciparum / MSH2 / ミスマッチDNA修復 / CRISPR/Cas9 / ミューテーター

Outline of Final Research Achievements

MSH2-1 is thought to be one of the major DNA mismatch repair proteins of Plasmodium species. The MSH2-1 mutant of P. falciparum 3D7 was generated by substituting the 513rd proline to threonine with CRISPR/Cas9 based gene editing. The parent strain 3D7 and the MSH2-1 mutant were maintained by the continuous in vitro culture for around 200 days, and then spontaneous mutations were genome-widely evaluated by high-throughput sequencing. Increases of insertion/deletion mutations in microsatellites and structural variants in subtelomeric regions were observed in the MSH2-1 mutant.

Free Research Field

寄生虫学