Analysis of the mannnoprotein that is involved in the host intestinal attachment in Candida glabrata

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K19090
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including mycology)
• Research Institution: Chiba University
• Principal Investigator: michiyo Sato 千葉大学, 真菌医学研究センター, 特任助教 (70525386)
• Research Collaborator: CHIBANA hiroji 千葉大学, 真菌医学研究センター, 准教授 ; TAKAHASHI azusa 千葉大学, 真菌医学研究センター, 技術職員
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: Candida glabrata / 病原性因子

Outline of Final Research Achievements

Candida pathogens cause serious opportunistic infections to immunodeficient patients. Candida glabrata is particular problem among Candida pathogens, because its frequency of the isolations from patients tends to increase year by year. In this study, to reveal the infection mechanism of Candida glabrata through the host intestinal mucosa, we performed the screening of the factor that is involved in the adhesion to the host intestinal mucosa. We constructed the screening system to select the gene-disrupted strains by analyzing the samples isolated from the intestinal mucosa of the mice which were ingested the library composed various gene-disrupted strains with a next-generation sequencer. Though we cannot identify the factor involved in the adhesion to the host intestinal mucosa, we identified one of the the enzyme for ergosterol biosynthesis as the virulence factor that can become the useful target for the development of antifungal drugs.

Free Research Field

分子生物学