Expression mechanism of three CsrB family regulatory RNA in Vibrio alginolyticus

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K19093
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including mycology)
• Research Institution: Okayama University
• Principal Investigator: Gotoh Kazuyoshi 岡山大学, 医歯薬学総合研究科, 助教 (20626593)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: Vibrio / 調節RNA

Outline of Final Research Achievements

Vibrio alginolyticus posses three CsrB family regulatory RNA. In this study, we investigated the mechanism of these RNAs expression system in transcriptional and post-transcriptional level. First, we checked stability among these RNAs using rifampicin RNA-chase assay. As a result, no any difference of RNA stability in three RNAs was shown. Next, we analyzed the possibility that ORF regions affect transcriptional activity. However, reporter assay indicated that the no difference of the activity was shown in ORF containing promoter. These resultu indicated that unknown mechanism produce the non-redundancy among three CsrB family regulatory RNAs.

Free Research Field

細菌学