2017 Fiscal Year Final Research Report
Analysis of MRSA evolution mechanism by generalized transduction
| Project/Area Number |
15K19095
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Azabu University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUZAKI Shigenobu (00190439)
KUROKAWA Kenji (80304963)
MIYAZAKI Naoyuki (00634677)
MURATA Kazuyoshi (20311201)
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| Research Collaborator |
UCHIYAMA Iyo
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | ファージ / 遺伝子の水平伝播 |
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| Outline of Final Research Achievements |
In this study, we analyzed the horizontal gene transfer in the environment, using the model of phage transduction of phage S6 in MRSA. First, analyzing phylogeny of phage based on the whole genome, phage S6 was related to the other phage infecting Gram-positive bacteria and that infecting Gram-negative bacteria, which suggested that there may be specific phage group with transducing ability. Second, phage S6 has ability to adsorb the Staphylococcus aureus cells via beta-glycosilation of wall teichoic acids on the cell wall. Third, phage S6 was considered to package the host DNA, transfer it to the new recipient cells, and the gene acquisition occurred by homologous recombination.
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| Free Research Field |
細菌学
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