Development of the toxoid vaccines and neutralizing monoclonal antibodies against Shiga toxins

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K19096
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including mycology)
• Research Institution: University of the Ryukyus
• Principal Investigator: HARAKUNI Tetsuya 琉球大学, 熱帯生物圏研究センター, 協力研究員 (60593598)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 腸管出血性大腸菌(EHEC) / 志賀毒素(Stx) / サブユニットワクチン / モノクロナル抗体 / リフォールディング / コイルドコイル分子 / ５量体

Outline of Final Research Achievements

Enterohemorrhagic E. coli (EHEC) produces two immunologically distinct types of Shiga toxin (Stx, ie, Stx1 and Stx2). EHEC toxoid vaccines should prevent intoxication caused by both types of toxins. Using the enterotoxin B subunit stabilization technology we established before, we developed the toxoid vaccines that sufficiently neutralized Stx1 and Stx2. In addition, we produced neutralizing monoclonal antibodies using the toxoid vaccines as an immunogen.

Free Research Field

細菌学(含真菌学)