2016 Fiscal Year Final Research Report
Biogenesis of lamellar body-like organelles in host cells expressing Mycobacterium tuberculosis PE_PGRS62
| Project/Area Number |
15K19102
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | National Center for Global Health and Medicine |
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Principal Investigator |
Iwai Hiroki 国立研究開発法人国立国際医療研究センター, その他部局等, 研究員(移行) (70443116)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 結核菌 |
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| Outline of Final Research Achievements |
PE_PGRS62 is a member of the M. tuberculosis PE_PGRS family of glycine-rich proteins, and thought to be a virulent factor. We described here that two type II alveolar epithelial cell (AEC II)-like cell lines, A549 and T7, expressing GFP-PE_PGRS62 possessed lamellar body-like vesicles. The vesicles contained lipid with multi-membrane structures. PE_PGRS62 was co-localized on the vesicles with the ATP-binding cassette transporter ABCA3, a marker of a lamellar body limiting membrane of AEC II. These results suggest that PE_PGRS62 generates lamellar body-like vesicles containing lipid in AEC II.
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| Free Research Field |
細菌学
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