2016 Fiscal Year Final Research Report
The role of PB2 segment on genome packaging of influenza A virus
Project/Area Number |
15K19104
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Virology
|
Research Institution | The University of Tokyo |
Principal Investigator |
Murakami Shin 東京大学, 大学院農学生命科学研究科(農学部), 助教 (10636757)
|
Research Collaborator |
NODA Takeshi 京都大学, ウイルス再生医学研究所, 教授
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Keywords | A型インフルエンザウイルス / ゲノムパッケージング |
Outline of Final Research Achievements |
By serial passaging of PB2 segment deficient virus (ΔPB2 virus) in PB2 stably expressing MDCK cells, we obtained 3 strains ofΔPB2 virus with high growth property. All the 3 strains possessed mutations in their NP proteins. The NP mutations caused sifting the charge to basic side. Although ΔHA virus lacked its HA segment, ΔHA virus possessed 8 genome segment in its virion. Next generation sequencing and Northern blotting analysis revealed that ΔHA virus incorporated host-derived rRNA in its virion. These findings highlight the importance of the assembly of eight RNPs in progeny virions for genome packaging.
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Free Research Field |
獣医ウイルス学
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