Study of the host factor important for secondary envelopment of HSV-1

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K19108
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: Tokyo Metropolitan Institute of Medical Science (2016); The University of Tokyo (2015)
• Principal Investigator: KOBAYASHI Kyousuke 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 主任研究員 (80644989)
• Research Collaborator: IBA Hideo 千葉大学, 真菌医学研究センター, 教授 ; SAGARA Hiroshi 東京大学, 医科学研究所, 助教
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: ヘルペスウイルス / miRNA / ゴルジ体 / エンベロープ形成

Outline of Final Research Achievements

miRNAs regulate gene expression post transcriptionally. MiR-199a-3p, a kind of miRNA, reportedly impairs replication of various viruses. These evidences suggested that miR-199a-3p targets host factors important for replication of various virus. We here aimed to identify such host factors targeted by miR-199a using Herpes simplex virus-1 (HSV-1) as a model system. We identified human ARHGAP21 as a target of both miR-199a-5p and miR-199a-3p. Our results suggested that this molecule regulate the function of the Golgi apparatus and secondary envelopment of HSV-1 at the trans cisternae of the Golgi apparatus.

Free Research Field

ウイルス学