Creation of drug susceptibility prediction system based on VNTR genotyping of Mycobacterium avium

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K19181
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: International University of Health and Welfare
• Principal Investigator: Tatano Yutaka 国際医療福祉大学, 薬学部, 講師 (70432614)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: Mycobacterium avium / VNTR / 薬剤感受性 / キノロン / 抗酸菌

Outline of Final Research Achievements

The purpose of this study is to establish a system that can predict drug susceptibility based on polymorphic tandem repeat sequence (VNTR) genotype of Mycobacterium avium complex (MAC). Significant differences in sensitivity to quinolone were observed among groups constructed based on the VNTR genotype of M. avium clinical isolates derived from Shimane, Tochigi, Osaka and Fukuoka. Hence, it was suggested that the quinolone sensitivity could be predicted from the VNTR genotype of the strain. However, it was shown that the M. avium strains derived from Fukuoka have a different genetic background compared to the strains derived from the other three areas. Multivariate analysis showed that, among the 15 VNTR regions (MATR-1 - 9, 11 - 16), MATR-3 region showed a significant association with quinolone susceptibility.

Free Research Field

微生物学