Development of immunoserological diagnostic methods for fungal-allergic patients inflood-damaged areas

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K19188
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: National Institute of Health Sciences
• Principal Investigator: MAIKO WATANABE 国立医薬品食品衛生研究所, 衛生微生物部, 室長 (00432013)
• Co-Investigator(Renkei-kenkyūsha): KUBOSAKI Atsutaka 国立医薬品食品衛生研究所, 衛生微生物部, 主任研究官 (30425673)
• Research Collaborator: KAMATA Yoichi ; TSURIKISAWA Naomi ; OSHIKATA Chiyako ; KIMURA Noritaka
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 水害被災住宅 / 室内真菌叢 / 抗原特異的IgE / 化学発光酵素免疫測定法

Outline of Final Research Achievements

We developed the Chemiluminescent Enzyme Immunoassay (CLEIA) method for the detection of anti-fungal allergen antibody(IgE) for fungal-allergic patients in flood-damaged areas, with Aspergillus species and their related species which are the most allergic fungi. Our results indicated that positive rates for several allergic fungi among the dwellers in flood-damaged areas were in the range of 7.0-15.1% by the CLEIA method.
Because the growth of mites in the housedust is well correlated with the growth of fungi, we acquired the anti-Der_f_1 and Der_1 monoclonal antibody to construct the detection method for allergen of the genus Dermatophagoides in the future study.

Free Research Field

衛生微生物学