The investigation of effects by antibiotics on spread of ESBL-producing bacteria worldwide.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K19255
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Hygiene and public health
• Research Institution: Osaka Institute of Public Health
• Principal Investigator: Yamaguchi Takahiro 地方独立行政法人 大阪健康安全基盤研究所, 微生物部, 研究員 (80553635)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: プラスミド / 耐性菌 / コリスチン耐性遺伝子

Outline of Final Research Achievements

We investigated the effect of antibiotics on horizontal plasmid transduction using ESBL/AmpC producing E. coli were isolated from meats and fish and shellfish in Vietnam. As a result of the study, it was shown that the number of colonies of the transconjugants can be increased by adding a certain concentration at the time of conjugation in tetracycline and ciprofloxacin. Next, studies on the colistin resistance gene of ESBL / AmpC producing E. coli 261 strains revealed that 62 strains have plasmid harboring mcr-1 or mcr-3 and that plasmids are propagated by horizontal transmission. In addition, genetic analysis was performed on the plasmid having the mcr gene and its details were clarified.

Free Research Field

微生物学