Analysis of association between genetic heterogeneity of HBV, and SNPs determined by Next-Generation-Sequencing and HCC development after the initiation of nucleos(t)ide analogue treatment

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K19345
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Osaka City University
• Principal Investigator: kozuka ritsuzo 大阪市立大学, 大学院医学研究科, 病院講師 (10726657)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: B型肝炎 / 核酸アナログ / 肝発癌 / 次世代シークエンス / ウイルス遺伝子変異 / 宿主遺伝子多型 / 肝硬変 / 肝線維化マーカー

Outline of Final Research Achievements

We investigated characteristics of hepatocellular carcinoma (HCC) development after the initiation of nucleos(t)ide analogue treatment in chronic hepatitis B patients from a viewpoint of clinical, viral, and host factor. First, liver cirrhosis status and high serum fibrosis marker levels at baseline were the significant risk factors of developing HCC. Second, we analyzed genetic heterogeneity of hepatitis B virus (HBV) by Next-Generation-Sequencing. Of the HBV full-length sequences, amino acid substitution in the polymerase region was significantly associated with HCC development. Third, we examined single nucleotide polymorphisms (SNP) by the Real-Time PCR Assay. Our results suggested that SNP in HLA gene was significantly associated with HCC development among 18 SNPs.

Free Research Field

肝臓学