Crosstalk of exosome between lung and bone marrow in lung fibrosis

2018 Fiscal Year Final Research Report

• Project/Area Number: 15K19432
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Respiratory organ internal medicine
• Research Institution: Nippon Medical School
• Principal Investigator: Minoru Inomata 日本医科大学, 大学院医学研究科, 研究生 (50465291)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Keywords: micro RNA / micro RNA 16 / C57BL/6 / bleomycin / anti-fibrotic effect

Outline of Final Research Achievements

Bleomycin was infused with osmotic pumps into C57BL/6 mice. Exosomes were extracted from serum of mice using ExoQuick. They were then, subjected to microarray analyses of each microRNA level in the exosomes using the miRNA microarray platform. In BLM-treated group, exosomal miR-16 increased by up to 10.32-fold on day 14 when compared with that in saline-treated group. MiR-16 mimic administration on day 14 after BLM-challenge significantly ameliorated pulmonary fibrosis. HFL-1 cells were pretreated with miR-16 mimic for 24 h before the addition of TGF-beta1. Treatment with miR-16 mimic significantly inhibited fibrotic marker analyzed by qRT-PCR and western blotting.

Free Research Field

呼吸器内科

Academic Significance and Societal Importance of the Research Achievements

肺線維化病態において、線維化病変由来のエクソソームには線維化を悪化させるmicro RNAが含まれていることが明らかとなり、特発性肺線維症の病態解明や新規治療薬の開発にもつながる可能性がある。