2016 Fiscal Year Final Research Report
Functional analysis for apical transporters by gene silencing in isolated and cultured renal proximal tubules.
| Project/Area Number |
15K19441
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Kidney internal medicine
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 水・電解質代謝学 / ナトリウム－プロトン交換輸送体 / ＮＨＥ |
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| Outline of Final Research Achievements |
We aimed to explore the detailed mechanisms of sodium dependent acid base transport in the apical side of renal proximal tubules (PTs). Cell pH measurement was performed in freshly isolated PTs with several inhibitors including EIPA and DIDS, or in cultured PTs which was gene silenced with siRNA. Consequently, we revealed the existence of DIDS-sensitive components other than Na/H exchanger 3 in the apical side of PTs, which was a possible key in species differences of sodium dependent acid base transport among mice, rats and humans.
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| Free Research Field |
腎臓内科学
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