Mechanical dissection of leukemic stem cell generation by using primate ES/iPS cell-derived hematopoietic stem/progenitor cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K19552
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: The University of Tokyo
• Principal Investigator: Kohara Hiroshi 東京大学, 医科学研究所, 特任講師 (40528733)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 白血病 / ES細胞 / iPS細胞

Outline of Final Research Achievements

Inducible gene expression cassette of oncogenes including KRAS G12V were successfully inserted into adeno-associated virus integration site 1 (AAVS1) locus of human ES cells and iPS cells by genome editing technique. CD34-positive hematopoietic cells were induced from these ES/iPS cells. After additional 7 day　culture of these hematopoietic cells with KRAS G12V induction resulted in altered cell growth. Now CD34-positive hematopoietic cells were transplanted into irradiated immune-deficient mice in order to investigate in vivo competitive repopulation ability in comparison with normal hematopoietic cells derived from ES/iPS cells.

Free Research Field

幹細胞生物学