The mechanism of drug resistance regulated by miR-155 in T-ALL

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K19557
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: Jichi Medical University
• Principal Investigator: Koyama Daisuke 自治医科大学, 医学部, 客員研究員 (50741071)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: miR-155 / bortezomib / T-ALL / 薬剤耐性

Outline of Final Research Achievements

We previously reported that bortezomib induces cell death and increases chemosensitivity via transcriptional repression of Notch1 in T-cell acute lymphoblastic leukemia. The down-regulation of Notch1 was caused by the degradation of Sp1. But the mechanism of Sp1 degradation is still unknown. We hypothesized that Sp1 is degraded by some microRNA. In order to find the microRNA targeting Sp1, we performed the microRNA array and identified that miR-155 was up-regulated by bortezomib. We confirmed it using CRISPR/Cas9 system. These results indicated that miR-155 could be the biomarker of the drug sensitivity for bortezomib.

Free Research Field

血液内科