Mechanism of combining antibacterial therapy led to enhancement of bactericidal activity and suppressing toxin production

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K19590
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Infectious disease medicine
• Research Institution: Keio University
• Principal Investigator: Morozumi Miyuki 慶應義塾大学, 医学部(信濃町), 講師 (40383559)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: Streptococcus agalactiae / 併用効果 / 殺菌作用の増強 / 併用メカニズム

Outline of Final Research Achievements

Combinational chemotherapy of β-lactam and aminoglycoside antibiotics, which are having different action targets, synergistically increased bactericidal activity against β-hemolytic streptococci and Streptococcus pneumoniae isolated from patients with invasive infection. Morphologic changes after exposure to the agents were observed by transmission electron microscopy. Obvious abnormal image was observed after exposure to β-lactam and/or aminoglycoside antibiotics. In addition, GM concentration in the cells was measured using LC/MS/MS, resulting GM concentrations in the cells increased when it was combined with β-lactam antibiotics than the alone.
These results suggest that combining β-lactam antibiotics with GM led to enhancement of bactericidal activity.

Free Research Field

細菌学