Construction of human artificial chromosome towards practical use

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K19615
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pediatrics
• Research Institution: Tottori University
• Principal Investigator: Uno Narumi 鳥取大学, 医学(系)研究科(研究院), 助教 (30733357)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ヒト人工染色体 / 微小核細胞融合法

Outline of Final Research Achievements

Human artificial chromosome vector (HAC) is promising for the development of gene- and cell-therapy for Duchenne muscular dystrophy, which is a genetic disorder with an X-linked recessive inheritance pattern . However, HAC should be developed and introduced to target cells in a condition without animal cells. In this study, we developed a chromosome modification technology for human chromosome in CHO or A9 cells of the model cells of somatic cells with CRISPR/Cas9, called genome editing tool. On the other hand, the chromosome modification technology in human cells was examined and chemical compounds was screened towards the development of microcell-mediated chromosome transferring with human cells as a donor of a chromosome. Although these studies did not showed an expected results, further consideration should be required towards the achievements of the development of these technologies.

Free Research Field

染色体工学