regenerative medicine of extensive trachea using a decellularization trachea administrated amniotic fluid-derived mesenchymal stem cell

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K19657
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Kyushu University
• Principal Investigator: Wada Momoko 九州大学, 医学研究院, 共同研究員 (30741219)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 気管再生 / 脱細胞化 / scaffold / 間葉系幹細胞

Outline of Final Research Achievements

A decellularization protocol for the trachea of the mouse was prepared according to the past papers and we made decellularized trachea. Although there was no cell remnant observed, the strength of the trachea was not preserved. That is why we revised the protocol and rebuilt decellularized trachea. It was succeeded in creating a decellularized trachea with strength maintained almost without observing the residual of the cell. We could create a minimum acceptable protocol and it can be a foothold for regeneration trachea creation.
Mesenchymal stem cells were set as a cell source for regenerating trachea, and a difference in cartilage differentiation ability was observed between donors. As a result of examination focusing on Epigenetics, we found a difference in chromatin structure, suggesting the possibility of being the cause of the difference in cartilage differentiation potential.

Free Research Field

小児外科