2016 Fiscal Year Final Research Report
Genetic expression analysis of circulating tumor cells in patients with melanoma
| Project/Area Number |
15K19683
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Dermatology
|
|---|
| Research Institution | Shinshu University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Keywords | メラノーマ / 循環腫瘍細胞 / 遺伝子発現 |
|---|
| Outline of Final Research Achievements |
In this study, we tried to identify and analyze circulating tumor cells (CTCs) from patients with melanoma. To this aim, we isolated peripheral blood mononuclear cells and stained them with HMW-MAA antibody and B7-H3 antibody. These antibodies combined with negative selection of white blood cells seemed useful for identification CTCs. DNA sequencing was revealed melanoma-specific gene mutation. However. RNA analysis was failed to evaluate mRNA expression. The major reason of this difficulty may be due to insufficient RNA quality during CTC isolation process.
|
| Free Research Field |
皮膚科
|
