2016 Fiscal Year Final Research Report
Development of folliclogenetic tecnique utilizing mesenchymal cells capable of controlling microenvironment
| Project/Area Number |
15K19703
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | Kyorin University |
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Principal Investigator |
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| Research Collaborator |
OHYAMA MANABU 杏林大学, 医学部, 教授 (10255424)
TSUKASHIMA AKI 杏林大学, 医学部, 実験助手
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 毛包再生 / 毛包新生 / 線維芽細胞 / 毛乳頭細胞 / FGF / WNTシグナル |
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| Outline of Final Research Achievements |
State of microenvironment affects the efficiency of hair follicle (HF) induction. In this study, we sought for a methodology to support human HF regeneration by regulating surrounding microenvironment. First, we confirmed that human-scalp derived fibroblasts (hsFBs) demonstrate unique FGF expression pattern and possess the capacity to modulate the profile in response to WNT activation represented by up-regulation of FGF9 and down-regulation of FGF7. Supplementation of these FGFs to HF composing cells resulted in significant alteration in representative HF marker gene expression. When FGF9 was administered to subcutaneously into immunodeficient mice, co-transplanted with mice epidermal and dermal cells, acceleration of HF induction and increase in the number and the diameter of newly formed HFs were observed. These results suggest that hsFBs may support HF formation by modulating FGF expression profile in response to regional WNT cue which can be utilized for human HF regeneration.
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| Free Research Field |
毛髪疾患
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