2016 Fiscal Year Final Research Report
Elucidation of the pathology and establishment of the therapy of thrombotic microangiopathy after liver transplantation - Development of a new complement measurement system -
| Project/Area Number |
15K19851
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 肝移植 / 補体 |
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| Outline of Final Research Achievements |
In order to elucidate the pathology of thrombotic microangiopathy after liver transplantation (TMA-LTx), We focused on the complement system, which is the most sensitive and rapid innate immunity. In this study, we developed a new complement measurement system, using Multiplex Assay and Cytometric Bead Array. Regarding ADAMTS13, which is one of the parameter of TMA-LTx, we could create a highly reliable test curve, but for the other antibodies, the measurement result was unstable though sufficient antibodies were bound to the beads. There was no particular problem in the method for preparing antibody beads. It is confirmed that a complement measurement system using Multiplex can be constructed by selecting an appropriate antibody.
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| Free Research Field |
肝移植
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