2016 Fiscal Year Final Research Report
Protecting liver sinusoidal endothelial cells suppresses apoptosis in acute liver damage
| Project/Area Number |
15K19870
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | Drug Delivery System / 肝類洞内皮細胞 / 肝細胞 / アポトーシス / SiRNA, Bax |
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| Outline of Final Research Achievements |
Bax siRNA was transfected into a sinusoidal endothelial cell line (M1) to suppress apoptosis. C57BL/6J mice were divided into three groups: (i) a control group, only intravenous saline; (ii) a nonselective group, injections of siRNA sealed in the nonselective DDS; and (iii) an LSEC-transfer efficient group, injections of siRNA sealed in an LSEC-transfer efficient
DDS. Bax siRNA had an anti-apoptotic effect on M1 cells. Silver impregnation staining indicated that the sinusoidal space was maintained in the LSEC-transfer efficient group but not in the other groups. Electron microscopy showed that the LSECs were slightly impaired, although the sinusoidal structure was maintained in the LSEC-transfer efficient group. Hepatocyte apoptosis was reduced by the efficient suppression of LSEC apoptosis with a novel DDS. Protecting the sinusoidal structure by suppressing LSEC damage will be an effective treatment for acute liver failure.
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| Free Research Field |
消化器外科学
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