2016 Fiscal Year Final Research Report
Therapeutic implication for cancer-associated fibroblasts (CAF) in cholangiocarcinoma
| Project/Area Number |
15K19900
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Kumamoto University |
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Principal Investigator |
IMAI Katsunori 熊本大学, 医学部附属病院, 助教 (60555746)
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| Research Collaborator |
OKABE Hirohisa 熊本大学, 医学部附属病院, 非常勤診療医師 (40573621)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 癌関連線維芽細胞（CAF） / 胃癌 / CXCL12 / CXCR4 |
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| Outline of Final Research Achievements |
Cancer associated fibroblasts (CAFs) were hardly isolated from human cholangiocarcinoma tissue samples because tissue was stiff and elastic. Instead, CAFs were isolated from human gastric cancer tissues. Activation of CAFs was confirmed by expression of SMA by immunocytochemistry. Three out of six human CAFs overexpressed CXCL12. Co-culture of CXCL12-expressing human CAF and gastric cancer cell line, OACM5.1C expressing CXCR4 induced invasive capacity of cancer cell. On the other hand, this interaction was prohibited by CXCR4 antagonist.
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| Free Research Field |
腫瘍免疫学
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