The development of the artificial heart valve having a growth potential with the aim of permanent engraftment

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K19919
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular surgery
• Research Institution: Osaka University
• Principal Investigator: Ozawa Hideto 大阪大学, 医学系研究科, 寄附講座助教 (50747699)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 先天性心疾患 / 組織工学 / 再生医療 / 心臓血管外科学

Outline of Final Research Achievements

We examined the recellularization process after fresh decellularized heart valve transplantation, in large animal experiments. METHODS: Pulmonary artery valves of heart transplant recipients removed during brain death heart transplantation were decellularized with 0.5% deoxycholic acid and 0.5% dodecyl sulfate. This valve was transplanted to the pulmonary valve position of the mini pig, and histological examination was carried out 6 months after transplantation. RESULTS: Migration of CD31, Vimentin-positive cells to the graft was observed, and the presence of cells produced cytokines such as VEGF, SDF-1 were observed and we also revealed the presence of the cells which were formed of myoblast. Theses findings suggest that new engraftment of decellularized heart valve was obtained.

Free Research Field

小児心臓血管外科学