Analysis of extracellular microRNA having protective effects against ischemia-perfusion injury of organs after ischemic preconditioning

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K19921
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular surgery
• Research Institution: Yamaguchi University
• Principal Investigator: UENO Koji 山口大学, 医学部附属病院, 助教 (30736070)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 細胞外microRNA / エクソソーム / 虚血プレコンディショニング

Outline of Final Research Achievements

Ischemic preconditioning (IPC) has protective effects against ischemia-perfusion injury of organs. Plasma vascular endothelial growth factor (VEGF) levels were increased 24 h after rIPC compared with those in sham-operated animals. Some microRNAs targeting VEGF were downregulated in exosomes 24 h after rIPC. The expression levels of miR-762 and miR-3072-5p targeting VEGF were downregulated in femoral CD34-positive bone marrow (BM) cells 24 h after rIPC compared with those in sham-operated controls. These data suggest that rIPC transiently increases plasma VEGF levels by downregulating miR-762 and miR-3072-5p in CD34-positive BM cells, leading to protection against organ ischemia.

Free Research Field

microRNA