Investigation for an effect of heart function by mixing neural cell for induced pluripotent stem cell derived cardiomyocyte using tissue engineering

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K19925
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular surgery
• Research Institution: Saga University
• Principal Investigator: Mukae Yosuke 佐賀大学, 医学部, 助教 (90746570)
• Research Collaborator: Morita Shigeki ; Furukawa Kojiro ; Itoh Manabu ; Noguchi Ryo ; Kitsuka Takahiro ; Nakayama Koichi ; Arai Ken-ichi ; Node Koichi ; Oyama Jun-ichi ; Toda Shuji
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: ヒトiPS細胞由来心筋細胞 / ヒトiPS細胞由来神経前駆細胞 / スフェロイド / 収縮率 / 心筋再生

Outline of Final Research Achievements

At first year, we could fabricate beating spheroids by mixing human induced pluripotent stem cell (hiPS) cell derived neural progenitors (hiPS-NPs) for hiPS cell derived cardiomyocyte. The second year, the ratio of hiPS-NPs was set at 0%, 10%, 20%, 30%, and 40% of the spheroids. These spheroids were observed and recorded. The only 40% of hiPS-NPs group stopped beating by 14 days after spheroid fabrication. The spheroids involving 30% of hiPS-NPs showed functional shortenings rather than the 40% of hiPS-NPs spheroids. These results mean that adding hiPS-NPs to cardiomyocyte can change the contractile function of cardiac spheroid. Furthermore, we demonstrated neuro-specific antibody positive structure in the spheroid which beated for two weeks by immunofluorescence study. This result indicated the possibility of differentiation of hiPS-NPs to neural fibers in the mixed spheroid.

Free Research Field

心筋再生