Exploration of targeting glutaminolysis as an anticancer strategy in lung squamous cell carcinoma

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K19931
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Respiratory surgery
• Research Institution: Niigata University
• Principal Investigator: Sato Seijiro 新潟大学, 医歯学系, 助教 (40646931)
• Co-Investigator(Renkei-kenkyūsha): SAIJO Yasuo 新潟大学, 大学院医歯学総合研究科・腫瘍内科学分野 (10270828)
• Research Collaborator: YE XULU
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: グルタミン代謝 / 肺扁平上皮癌 / グルタミナーゼ1 / グルタミナーゼ2

Outline of Final Research Achievements

Five of six lung squamous cell carcinoma cell lines exhibited glutamine- dependence. The extent of dependence was correlated with the mRNA levels of GLS1/GLS2. The
proliferation of Sq-1, LK-2, LC-1/sq, EBC-1 and RERF-LCAI cells was significantly decreased in Gln-depleted medium. In contrast, no significant inhibition of cell proliferation was observed in the QG56 cell line at either 72 or 120 h.
When mRNA expression ratio of GLS1/GLS2 is essential for Gln dependence in lung squamous cell carcinoma cell lines, the relative mRNA level of GLS1 was markedly higher than that of GLS2 in all cell lines. We found that GLS1 may be also essential to glutaminolysis in lung squamous cell carcinoma cell lines.

Free Research Field

呼吸器外科