Challenge for pulmonary reproduction after lung resection by the paracrine effect of growth factors secreted from mesenchymal stem cell sheets

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K19940
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Respiratory surgery
• Research Institution: Yamaguchi University
• Principal Investigator: MURAKAMI Junichi 山口大学, 医学部附属病院, 助教 (10725683)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 代償性肺成長 / 間葉系幹細胞 / 低酸素プレコンディショニング

Outline of Final Research Achievements

We focused on mesenchymal stem cells seem to secrete growth factors related to compensatory growth of the remaining lung after pneumonectomy and analyzed the characteristics of them. Consequently, it was shown that rabbit bone marrow-derived mesenchymal stem cells possessed the capacity to differentiate into osteoblasts, chondrocytes, adipocytes lineages. Hypoxic preconditioning (2% 02) increased secretion of vascular endothelial growth factor (VEGF) in mesenchymal stem cell sheets. Furthermore, the conditioned medium augmented tube formation of human vascular endothelial cells and decreased cardiac fibroblast migration.

Free Research Field

呼吸器外科