Epigenetic analysis of moyamoya disease

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K19961
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurosurgery
• Research Institution: Nagoya University
• Principal Investigator: ARAKI Yoshio 名古屋大学, 医学部附属病院, 病院講師 (80467290)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: もやもや病 / エピジェネティクス / マイクロアレイ

Outline of Final Research Achievements

In this study, we have tried (1) to perform comprehensive expression analysis using microarray with RNA samples extracted from intracranial and extracranial vessels of Moyamoya disease patients, (2) to verify the relationship with RNF 213 and PENK 143-183, which was identified by cerebrospinal fluid exhaustive analysis using SELDI-TOF-MS, (3) to identify specific genes involved in the development of Moyamoya disease based on transcriptional regulatory mechanism and pathway analysis by epigenetics analysis, finally (4) o create an animal model of Moyamoya disease. It was difficult to establish a methodology for RNA extraction from microscopic specimens of intracranial and extracranial blood vessels while maintaining sufficient quality. On the other hand, we performed expression analysis using microarray for RNA contained in cerebrospinal fluid of the patients with Moyamoya disease and the others, but eventually no significant results were obtained.

Free Research Field

脳神経外科