The analysis of expression of treatment-related genes and glioma stem cells in the hypoxic region of glioblastoma.

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K19977
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurosurgery
• Research Institution: Yokohama City University
• Principal Investigator: OHTAKE Makoto 横浜市立大学, 附属病院, 指導診療医 (70644607)
• Research Collaborator: KAWAHARA Nobutaka ; MURATA Hidetoshi ; UMEMURA Masanari
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 神経膠芽腫 / 低酸素領域 / CuATSM / 幹細胞 / MGMT遺伝子

Outline of Final Research Achievements

Glioblastoma is the most common and most aggresive tumor in adults. One of the factors of treatment resistence is the existence of hypoxic regions located in the tumor. Cu-ATSM (Cu-diacetyl-bis(N4-methylthiosemicarbazone)) was developed as a hypoxic tracer in PET. Our previous reports revealed a relationship between Cu-ATSM uptake and hypoxic marker expression. If the treatment resistent factors are mainly existed in the hypoxic regions of glioblastoma, the safe treatment targeted the hypoxic regions such as stereotactic strategy becomes possible. In this study, we took the hypoxix tissue at the surgery, and performed immunopathological examinations and gene analysis. Moreover, we examined the target therapy for Cu-ATSM high uptake regions. Now we follow up the clinical course and examine the relationship of tissue status and clinical outcome.

Free Research Field

脳腫瘍